PrimePCR™ SYBR® Green Assay: CYB5R3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: CYB5R3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CYB5R3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009335
Assay Design:   Intron-spanning
Chromosome Location:   22:43015907-43019861question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000352397 ENST00000407623 ENST00000407332 ENST00000402438 ENST00000361740 ENST00000396303

Gene Information

This gene encodes cytochrome b5 reductase which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids in cholesterol biosynthesis and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. [provided by RefSeq Jan 2010]

Gene Symbol:   CYB5R3
Gene Name:   cytochrome b5 reductase 3
Aliases:   B5R, DIA1
RefSeq:   NC_000022.10 NG_012194.1 NT_011520.12
Ensembl:   ENSG00000100243
Entrez:   1727
Chromosome Mapping:   22q13.31-qter,22q13.2-q13.31

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