PrimePCR™ SYBR® Green Assay: PLN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030625

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PLN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PLN, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016488
Assay Design:   Intron-spanning
Chromosome Location:   6:118869495-118880055question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000357525

Gene Information

The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure. [provided by RefSeq Jul 2008]

Gene Symbol:   PLN
Gene Name:   phospholamban
Aliases:   CMD1P, PLB
RefSeq:   NC_000006.11 NG_009082.1 NG_021248.1 NT_025741.15
Ensembl:   ENSG00000198523
Entrez:   5350
Chromosome Mapping:   6q22.1

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