PrimePCR™ SYBR® Green Assay: Relb, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Relb, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Relb, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCED0007594
Assay Design:		exonic
Chromosome Location:		1:81797216-81799290question
Amplicon Length:		90
Splice Variants Targeted:		ENSRNOT00000046486 ENSRNOT00000002881 ENSRNOT00000011956 ENSRNOT00000006527 ENSRNOT00000035545 ENSRNOT00000025570 ENSRNOT00000020950 ENSRNOT00000027789 ENSRNOT00000074659 ENSRNOT00000026843 ENSRNOT00000003452 ENSRNOT00000035309 ENSRNOT00000034041 ENSRNOT00000017495 ENSRNOT00000022351 ENSRNOT00000009269 ENSRNOT00000035597 ENSRNOT00000046563 ENSRNOT00000000200 ENSRNOT00000066542 ENSRNOT00000073209 ENSRNOT00000020147 ENSRNOT00000002929 ENSRNOT00000040206 ENSRNOT00000047836 ENSRNOT00000065373 ENSRNO

Gene Information

Description Not Available

Gene Symbol:		Relb
Gene Name:		Avian reticuloendotheliosis viral (v-rel) oncogene related B
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSRNOG00000033235
Entrez:		100360982
Chromosome Mapping:		1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	36.540000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.