PrimePCR™ SYBR® Green Assay: ABP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0025018

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ABP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ABP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004394
Assay Design:   Exonic
Chromosome Location:   7:150553714-150553812question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000460213 ENST00000360937 ENST00000416793 ENST00000483043 ENST00000487631 ENST00000493429 ENST00000467291

Gene Information

This gene encodes a membrane glycoprotein that is expressed in many epithelium-rich and/or hematopoietic tissues and oxidatively deaminates putrescine and histamine. The protein may play a role in controlling the level of histamine and/or putrescine in these tissues. It also binds to and is inhibited by amiloride a diuretic that acts by closing epithelial sodium ion channels. [provided by RefSeq Jul 2008]

Gene Symbol:   ABP1
Gene Name:   amiloride binding protein 1 (amine oxidase (copper-containing))
Aliases:   ABP, AOC1, DAO, DAO1, KAO
RefSeq:   NC_000007.13 NT_007914.15
Ensembl:   ENSG00000002726
Entrez:   26
Chromosome Mapping:   7q34-q36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.200000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download