PrimePCR™ SYBR® Green Assay: RP11-540D14.8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040261

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RP11-540D14.8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RP11-540D14.8, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037538
Assay Design:   exonic
Chromosome Location:   1:155058986-155059155question
Amplicon Length:   140
Splice Variants Targeted:   ENST00000374782 ENST00000269392 ENST00000450824 ENST00000575907 ENST00000418259 ENST00000377264 ENST00000421419 ENST00000423218 ENST00000315757 ENST00000399298 ENST00000368408 ENST00000556931 ENST00000418360 ENST00000505139

Gene Information

Description Not Available

Gene Symbol:   RP11-540D14.8
Gene Name:   Ephrin-A3; Uncharacterized protein; Uncharacterized protein; cDNA FLJ57652, highly similar to Ephrin-A3
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000251246
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.100000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download