PrimePCR™ SYBR® Green Assay: CRP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051721


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PrimePCR™ PreAmp for SYBR® Green Assay: CRP, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CRP, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0044459
Assay Design:		exonic
Chromosome Location:		1:159683831-159684217question
Amplicon Length:		71
Splice Variants Targeted:		ENST00000255030 ENST00000368112 ENST00000368111 ENST00000368110 ENST00000343919

Gene Information

The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently the level of this protein in plasma increases greatly during acute phase response to tissue injury infection or other inflammatory stimuli. [provided by RefSeq Sep 2009]

Gene Symbol:		CRP
Gene Name:		C-reactive protein, pentraxin-related
Aliases:		MGC149895, MGC88244, PTX1
RefSeq:		NC_000001.10 NG_013007.1 NT_004487.19
Ensembl:		ENSG00000132693
Entrez:		1401
Chromosome Mapping:		1q21-q23

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999700
y-intercept	36.160000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.