PrimePCR™ SYBR® Green Assay: POT1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026505

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: POT1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: POT1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0007539
Assay Design:   Intron-spanning
Chromosome Location:   7:124499088-124503454question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000357628 ENST00000393329 ENST00000451720 ENST00000265391 ENST00000440969 ENST00000393326

Gene Information

This gene is a member of the telombin family and encodes a nuclear protein involved in telomere maintenance. Specifically this protein functions as a member of a multi-protein complex that binds to the TTAGGG repeats of telomeres regulating telomere length and protecting chromosome ends from illegitimate recombination catastrophic chromosome instability and abnormal chromosome segregation. Increased transcriptional expression of this gene is associated with stomach carcinogenesis and its progression. Alternatively spliced transcript variants have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   POT1
Gene Name:   protection of telomeres 1 homolog (S. pombe)
Aliases:   DKFZp586D211, HPOT1
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000128513
Entrez:   25913
Chromosome Mapping:   7q31.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996700
y-intercept 34.050000
Efficiency 109

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download