PrimePCR™ SYBR® Green Assay: DIAPH3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DIAPH3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DIAPH3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021339
Assay Design:   Intron-spanning
Chromosome Location:   13:60545216-60548575question
Amplicon Length:   139
Splice Variants Targeted:   ENST00000400324 ENST00000377908 ENST00000400319 ENST00000400320 ENST00000267215 ENST00000400330 ENST00000400327 ENST00000413168 ENST00000400329 ENST00000267214 ENST00000453990

Gene Information

Description Not Available

Gene Symbol:   DIAPH3
Gene Name:   diaphanous homolog 3 (Drosophila)
Aliases:   AN, AUNA1, DKFZp434C0931, DKFZp686A13178, DRF3, FLJ34705, NSDAN, diap3, mDia2
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000139734
Entrez:   81624
Chromosome Mapping:   13q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 35.210000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download