PrimePCR™ SYBR® Green Assay: EIF2S2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0024621


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PrimePCR™ PreAmp for SYBR® Green Assay: EIF2S2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: EIF2S2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0003200
Assay Design:		Exonic
Chromosome Location:		20:32676667-32676820question
Amplicon Length:		124
Splice Variants Targeted:		ENST00000374980

Gene Information

Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits alpha beta and gamma with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP which recycles the EIF-2 complex for another round of initiation. [provided by RefSeq Jul 2008]

Gene Symbol:		EIF2S2
Gene Name:		eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa
Aliases:		DKFZp686L18198, EIF2, EIF2B, EIF2beta, MGC8508
RefSeq:		NC_000020.10 NT_011362.10
Ensembl:		ENSG00000125977
Entrez:		8894
Chromosome Mapping:		20pter-q12

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999400
y-intercept	35.570000
Efficiency	95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.