PrimePCR™ SYBR® Green Assay: APH1A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0025677

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: APH1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: APH1A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0007820
Assay Design:   Exonic
Chromosome Location:   1:150239772-150239873question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000369109 ENST00000360244 ENST00000236017 ENST00000414276

Gene Information

This gene encodes a component of the gamma secretase complex that cleaves integral membrane proteins such as Notch receptors and beta-amyloid precursor protein. The gamma secretase complex contains this gene product or the paralogous anterior pharynx defective 1 homolog B (APH1B) along with the presenilin nicastrin and presenilin enhancer-2 proteins. The precise function of this seven-transmembrane-domain protein is unknown though it is suspected of facilitating the association of nicastrin and presenilin in the gamma secretase complex as well as interacting with substrates of the gamma secretase complex prior to their proteolytic processing. Polymorphisms in a promoter region of this gene have been associated with an increased risk for developing sporadic Alzheimer's disease. Alternative splicing results in multiple protein-coding and non-protein-coding transcript variants. [provided by RefSeq Aug 2011]

Gene Symbol:   APH1A
Gene Name:   anterior pharynx defective 1 homolog A (C. elegans)
Aliases:   6530402N02Rik, APH-1, APH-1A, CGI-78
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000117362
Entrez:   51107
Chromosome Mapping:   1p36.13-q31.3

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