PrimePCR™ SYBR® Green Assay: AKR7A2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041619

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AKR7A2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AKR7A2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038897
Assay Design:   exonic
Chromosome Location:   1:19630484-19630627question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000235835 ENST00000330072

Gene Information

The protein encoded by this gene belongs to the aldo/keto reductase (AKR) superfamily and AKR7 family which are involved in the detoxification of aldehydes and ketones. The AKR7 family consists of 3 genes that are present in a cluster on the p arm of chromosome 1. This protein thought to be localized in the golgi catalyzes the NADPH-dependent reduction of succinic semialdehyde to the endogenous neuromodulator gamma-hydroxybutyrate. It may also function as a detoxication enzyme in the reduction of aflatoxin B1 and 2-carboxybenzaldehyde. [provided by RefSeq Oct 2011]

Gene Symbol:   AKR7A2
Gene Name:   aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase)
Aliases:   AFAR, AFAR1, AFB1-AR1, AKR7
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000053371
Entrez:   8574
Chromosome Mapping:   1p36.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.470000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download