PrimePCR™ SYBR® Green Assay: ACD, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050214

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ACD, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ACD, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042952
Assay Design:   exonic
Chromosome Location:   16:67692880-67693146question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000219251 ENST00000602382 ENST00000393919 ENST00000602320 ENST00000602850

Gene Information

This gene encodes a protein that is involved in telomere function. This protein is one of six core proteins in the telosome/shelterin telomeric complex which functions to maintain telomere length and to protect telomere ends. Through its interaction with other components this protein plays a key role in the assembly and stabilization of this complex and it mediates the access of telomerase to the telomere. Multiple transcript variants encoding different isoforms have been found for this gene. This gene which is also referred to as TPP1 is distinct from the unrelated TPP1 gene on chromosome 11 which encodes tripeptidyl-peptidase I. [provided by RefSeq Jul 2008]

Gene Symbol:   ACD
Gene Name:   adrenocortical dysplasia homolog (mouse)
Aliases:   PIP1, PTOP, TINT1, TPP1
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000102977
Entrez:   65057
Chromosome Mapping:   16q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.410000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download