PrimePCR™ SYBR® Green Assay: YWHAE, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050518

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: YWHAE, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: YWHAE, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043256
Assay Design:   exonic
Chromosome Location:   17:1303388-1303495question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000264335 ENST00000571732 ENST00000573026 ENST00000575977

Gene Information

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases RAF1 and IRS1 proteins suggesting its role in diverse biochemical activities related to signal transduction such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants one protein-coding and the other non-protein-coding have been found for this gene. [provided by RefSeq Aug 2008]

Gene Symbol:   YWHAE
Gene Name:   tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
Aliases:   14-3-3E, FLJ45465, FLJ53559, KCIP-1, MDCR, MDS
RefSeq:   NC_000017.10 NG_009233.1 NT_010718.16
Ensembl:   ENSG00000108953
Entrez:   7531
Chromosome Mapping:   17p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.580000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download