PrimePCR™ SYBR® Green Assay: RAB11FIP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052998

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RAB11FIP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RAB11FIP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045737
Assay Design:   exonic
Chromosome Location:   8:37732066-37732160question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000287263 ENST00000330843 ENST00000522727 ENST00000524118

Gene Information

Proteins of the large Rab GTPase family (see RAB1A; MIM 179508) have regulatory roles in the formation targeting and fusion of intracellular transport vesicles. RAB11FIP1 is one of many proteins that interact with and regulate Rab GTPases (Hales et al. 2001 [PubMed 11495908]).[supplied by OMIM Mar 2008]

Gene Symbol:   RAB11FIP1
Gene Name:   RAB11 family interacting protein 1 (class I)
Aliases:   DKFZp686E2214, FLJ22524, FLJ22622, MGC78448, NOEL1A, RCP, rab11-FIP1
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000156675
Entrez:   80223
Chromosome Mapping:   8p11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.480000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download