PrimePCR™ SYBR® Green Assay: MAPK13, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0053002


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PrimePCR™ PreAmp for SYBR® Green Assay: MAPK13, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: MAPK13, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0045741
Assay Design:		exonic
Chromosome Location:		6:36098469-36099136question
Amplicon Length:		69
Splice Variants Targeted:		ENST00000211287 ENST00000373759 ENST00000373761 ENST00000373766

Gene Information

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation differentiation transcription regulation and development. This kinase is closely related to p38 MAP kinase both of which can be activated by proinflammatory cytokines and cellular stress. MAP kinase kinases 3 and 6 can phosphorylate and activate this kinase. Transcription factor ATF2 and microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase. [provided by RefSeq Jul 2008]

Gene Symbol:		MAPK13
Gene Name:		mitogen-activated protein kinase 13
Aliases:		MGC99536, PRKM13, SAPK4, p38delta
RefSeq:		NC_000006.11 NT_007592.15
Ensembl:		ENSG00000156711
Entrez:		5603
Chromosome Mapping:		6p21.31

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999400
y-intercept	36.430000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.