PrimePCR™ SYBR® Green Assay: MX1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0053041

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PrimePCR™ PreAmp for SYBR® Green Assay: MX1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MX1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045780
Assay Design:   exonic
Chromosome Location:   21:42799698-42802462question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000398600 ENST00000413778 ENST00000419044 ENST00000398598 ENST00000424365 ENST00000417963 ENST00000441677 ENST00000455164 ENST00000380916 ENST00000336751 ENST00000375765 ENST00000375254 ENST00000375267 ENST00000375217 ENST00000375226 ENST00000579423

Gene Information

In mouse the interferon-inducible Mx protein is responsible for a specific antiviral state against influenza virus infection. The protein encoded by this gene is similar to the mouse protein as determined by its antigenic relatedness induction conditions physicochemical properties and amino acid analysis. This cytoplasmic protein is a member of both the dynamin family and the family of large GTPases. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jan 2009]

Gene Symbol:   MX1
Gene Name:   myxovirus (influenza virus) resistance 1, interferon-inducible protein p78 (mouse)
Aliases:   IFI-78K, IFI78, MX, MxA
RefSeq:   NC_000021.8 NG_027788.1 NT_011512.11
Ensembl:   ENSG00000157601
Entrez:   4599
Chromosome Mapping:   21q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.980000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download