PrimePCR™ SYBR® Green Assay: CDC25C, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0053071


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PrimePCR™ PreAmp for SYBR® Green Assay: CDC25C, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CDC25C, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0045810
Assay Design:		exonic
Chromosome Location:		5:137665335-137666790question
Amplicon Length:		87
Splice Variants Targeted:		ENST00000348983 ENST00000357274 ENST00000356505 ENST00000510119 ENST00000503022 ENST00000514555 ENST00000513970 ENST00000415130 ENST00000323760

Gene Information

This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described however the full-length nature of many of them is not known. [provided by RefSeq Jul 2008]

Gene Symbol:		CDC25C
Gene Name:		cell division cycle 25 homolog C (S. pombe)
Aliases:		CDC25
RefSeq:		NC_000005.9 NT_034772.6
Ensembl:		ENSG00000158402
Entrez:		995
Chromosome Mapping:		5q31

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998300
y-intercept	35.790000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.