PrimePCR™ SYBR® Green Assay: CRADD, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0053890


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PrimePCR™ PreAmp for SYBR® Green Assay: CRADD, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CRADD, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0046629
Assay Design:		exonic
Chromosome Location:		12:94072648-94072750question
Amplicon Length:		73
Splice Variants Targeted:		ENST00000552983 ENST00000332896 ENST00000552033 ENST00000548483 ENST00000542893 ENST00000541813

Gene Information

The protein encoded by this gene is a death domain (CARD/DD)-containing protein and has been shown to induce cell apoptosis. Through its CARD domain this protein interacts with and thus recruits caspase 2/ICH1 to the cell death signal transduction complex that includes tumor necrosis factor receptor 1 (TNFR1A) RIPK1/RIP kinase and numbers of other CARD domain-containing proteins. [provided by RefSeq Jul 2008]

Gene Symbol:		CRADD
Gene Name:		CASP2 and RIPK1 domain containing adaptor with death domain
Aliases:		MGC9163, RAIDD
RefSeq:		NC_000012.11 NT_029419.12
Ensembl:		ENSG00000169372
Entrez:		8738
Chromosome Mapping:		12q21.33-q23.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998600
y-intercept	36.910000
Efficiency	94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.