PrimePCR™ SYBR® Green Assay: BCAR1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057512


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PrimePCR™ PreAmp for SYBR® Green Assay: BCAR1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: BCAR1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0056407
Assay Design:		exonic
Chromosome Location:		16:75276810-75276946question
Amplicon Length:		107
Splice Variants Targeted:		ENST00000345512 ENST00000373779 ENST00000428026 ENST00000460170 ENST00000356870 ENST00000323874 ENST00000162330 ENST00000393422 ENST00000535626 ENST00000538440 ENST00000418647 ENST00000542031 ENST00000420641 ENST00000393420 ENST00000568864 ENST00000564028 ENST00000566465 ENST00000569006 ENST00000561970 ENST00000563323 ENST00000567215 ENST00000546196

Gene Information

BCAR1 or CAS is an Src (MIM 190090) family kinase substrate involved in various cellular events including migration survival transformation and invasion (Sawada et al. 2006 [PubMed 17129785]).[supplied by OMIM May 2009]

Gene Symbol:		BCAR1
Gene Name:		breast cancer anti-estrogen resistance 1
Aliases:		CAS, CAS1, CASS1, CRKAS, FLJ12176, FLJ45059, P130Cas
RefSeq:		NC_000016.9 NT_010498.15
Ensembl:		ENSG00000050820
Entrez:		9564
Chromosome Mapping:		16q23.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998400
y-intercept	35.650000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.