PrimePCR™ SYBR® Green Assay: FASN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0026813


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PrimePCR™ PreAmp for SYBR® Green Assay: FASN, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: FASN, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0008460
Assay Design:		Intron-spanning
Chromosome Location:		17:80051600-80053255question
Amplicon Length:		78
Splice Variants Targeted:		ENST00000306749 ENST00000545909

Gene Information

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA in the presence of NADPH into long-chain saturated fatty acids. In some cancer cell lines this protein has been found to be fused with estrogen receptor-alpha (ER-alpha) in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq Jul 2008]

Gene Symbol:		FASN
Gene Name:		fatty acid synthase
Aliases:		FAS, MGC14367, MGC15706, OA-519, SDR27X1
RefSeq:		NC_000017.10 NT_010663.15
Ensembl:		ENSG00000169710
Entrez:		2194
Chromosome Mapping:		17q25

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998000
y-intercept	35.400000
Efficiency	96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.