PrimePCR™ SYBR® Green Assay: RAPGEF4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027063

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RAPGEF4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RAPGEF4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009146
Assay Design:   Intron-spanning
Chromosome Location:   2:173913346-173916479question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000397081 ENST00000409036 ENST00000397087 ENST00000264111 ENST00000538974 ENST00000540783 ENST00000539331 ENST00000535187

Gene Information

Description Not Available

Gene Symbol:   RAPGEF4
Gene Name:   Rap guanine nucleotide exchange factor (GEF) 4
Aliases:   CAMP-GEFII, CGEF2, EPAC2, Nbla00496
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000091428
Entrez:   11069
Chromosome Mapping:   2q31-q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.040000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download