PrimePCR™ SYBR® Green Assay: DFFB, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

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PrimePCR™ PreAmp for SYBR® Green Assay: DFFB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DFFB, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010170
Assay Design:   Intron-spanning
Chromosome Location:   1:3786229-3789079question
Amplicon Length:   125
Splice Variants Targeted:   ENST00000378209 ENST00000339350 ENST00000378206 ENST00000338895 ENST00000430539

Gene Information

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   DFFB
Gene Name:   DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
Aliases:   CAD, CPAN, DFF-40, DFF2, DFF40
RefSeq:   NC_000001.10 NT_004350.19
Ensembl:   ENSG00000169598
Entrez:   1677
Chromosome Mapping:   1p36.3

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