PrimePCR™ SYBR® Green Assay: ARHGEF10L, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028144

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ARHGEF10L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ARHGEF10L, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011708
Assay Design:   Intron-spanning
Chromosome Location:   1:17964437-17965096question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000361221 ENST00000375415 ENST00000375408 ENST00000452522 ENST00000434513 ENST00000375420 ENST00000457829 ENST00000167825

Gene Information

ARHGEF10L is a member of the RhoGEF family of guanine nucleotide exchange factors (GEFs) that activate Rho GTPases (Winkler et al. 2005 [PubMed 16112081]).[supplied by OMIM Dec 2008]

Gene Symbol:   ARHGEF10L
Gene Name:   Rho guanine nucleotide exchange factor (GEF) 10-like
Aliases:   FLJ10521, GrinchGEF, KIAA1626
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000074964
Entrez:   55160
Chromosome Mapping:   1p36.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 33.830000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download