PrimePCR™ SYBR® Green Assay: KALRN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage


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PrimePCR™ PreAmp for SYBR® Green Assay: KALRN, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: KALRN, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0011901
Assay Design:		Intron-spanning
Chromosome Location:		3:124390706-124393292question
Amplicon Length:		92
Splice Variants Targeted:		ENST00000360013 ENST00000428018 ENST00000354186 ENST00000393496 ENST00000291478 ENST00000459915

Gene Information

Huntington's disease (HD) a neurodegenerative disorder characterized by loss of striatal neurons is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that interacts with the huntingtin-associated protein 1 which is a huntingtin binding protein that may function in vesicle trafficking. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq Jul 2008]

Gene Symbol:		KALRN
Gene Name:		kalirin, RhoGEF kinase
Aliases:		ARHGEF24, DUET, DUO, FLJ12332, FLJ16443, FLJ18196, FLJ18623, HAPIP, TRAD
RefSeq:		NC_000003.11 NG_012742.1 NT_005612.16
Ensembl:		ENSG00000160145
Entrez:		8997
Chromosome Mapping:		3q21.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999200
y-intercept	34.080000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.