PrimePCR™ SYBR® Green Assay: NUMA1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage


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PrimePCR™ PreAmp for SYBR® Green Assay: NUMA1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: NUMA1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0012027
Assay Design:		Intron-spanning
Chromosome Location:		11:71740298-71780891question
Amplicon Length:		86
Splice Variants Targeted:		ENST00000351960 ENST00000358965 ENST00000393695 ENST00000542977 ENST00000537217 ENST00000544238 ENST00000543937 ENST00000543009 ENST00000537930 ENST00000544129 ENST00000535947 ENST00000535087 ENST00000368959 ENST00000541719 ENST00000366394 ENST00000541641 ENST00000535111 ENST00000535838 ENST00000546131 ENST00000359652 ENST00000536544

Gene Information

Description Not Available

Gene Symbol:		NUMA1
Gene Name:		nuclear mitotic apparatus protein 1
Aliases:		NUMA
RefSeq:		NG_021423.1 NC_000011.9 NT_167190.1
Ensembl:		ENSG00000137497
Entrez:		4926
Chromosome Mapping:		11q13

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998900
y-intercept	34.970000
Efficiency	104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.