PrimePCR™ SYBR® Green Assay: IRS2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028619

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: IRS2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: IRS2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012831
Assay Design:   Intron-spanning
Chromosome Location:   13:110408626-110434464question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000375856

Gene Information

This gene encodes the insulin receptor substrate 2 a cytoplasmic signaling molecule that mediates effects of insulin insulin-like growth factor 1 and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. [provided by RefSeq Jul 2008]

Gene Symbol:   IRS2
Gene Name:   insulin receptor substrate 2
Aliases:   IRS-2
RefSeq:   NC_000013.10 NG_008154.1 NT_009952.14
Ensembl:   ENSG00000185950
Entrez:   8660
Chromosome Mapping:   13q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.310000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download