PrimePCR™ SYBR® Green Assay: ITGA2B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0028772


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PrimePCR™ PreAmp for SYBR® Green Assay: ITGA2B, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: ITGA2B, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0013206
Assay Design:		Intron-spanning
Chromosome Location:		17:42453686-42454456question
Amplicon Length:		121
Splice Variants Targeted:		ENST00000262407 ENST00000353281

Gene Information

ITGA2B encodes integrin alpha chain 2b. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibronectin receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. In addition to adhesion integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq Jul 2008]

Gene Symbol:		ITGA2B
Gene Name:		integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)
Aliases:		CD41, CD41B, GP2B, GPIIb, GTA, HPA3
RefSeq:		NC_000017.10 NG_008331.1 NT_010783.15
Ensembl:		ENSG00000005961
Entrez:		3674
Chromosome Mapping:		17q21.32

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998500
y-intercept	35.440000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.