PrimePCR™ SYBR® Green Assay: SP100, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best efficiency


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PrimePCR™ PreAmp for SYBR® Green Assay: SP100, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SP100, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0013310
Assay Design:		Intron-spanning
Chromosome Location:		2:231314415-231326035question
Amplicon Length:		124
Splice Variants Targeted:		ENST00000264052 ENST00000409824 ENST00000409341 ENST00000409112 ENST00000340126 ENST00000409897 ENST00000427101 ENST00000341950 ENST00000414648

Gene Information

This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis immunity and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein. [provided by RefSeq Aug 2011]

Gene Symbol:		SP100
Gene Name:		SP100 nuclear antigen
Aliases:		DKFZp686E07254, FLJ00340, FLJ34579
RefSeq:		NC_000002.11 NT_005403.17
Ensembl:		ENSG00000067066
Entrez:		6672
Chromosome Mapping:		2q37.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999700
y-intercept	34.400000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.