PrimePCR™ SYBR® Green Assay: NR1H3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028960

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NR1H3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NR1H3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013633
Assay Design:   Intron-spanning
Chromosome Location:   11:47283476-47289565question
Amplicon Length:   166
Splice Variants Targeted:   ENST00000395397 ENST00000405576 ENST00000481889 ENST00000531660 ENST00000407404 ENST00000441012 ENST00000467728 ENST00000405853 ENST00000527949

Gene Information

The protein encoded by this gene belongs to the NR1 subfamily of the nuclear receptor superfamily. The NR1 family members are key regulators of macrophage function controlling transcriptional programs involved in lipid homeostasis and inflammation. This protein is highly expressed in visceral organs including liver kidney and intestine. It forms a heterodimer with retinoid X receptor (RXR) and regulates expression of target genes containing retinoid response elements. Studies in mice lacking this gene suggest that it may play an important role in the regulation of cholesterol homeostasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   NR1H3
Gene Name:   nuclear receptor subfamily 1, group H, member 3
Aliases:   LXR-a, LXRA, RLD-1
RefSeq:   NC_000011.9 NT_009237.18 NG_023296.1
Ensembl:   ENSG00000025434
Entrez:   10062
Chromosome Mapping:   11p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.090000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download