PrimePCR™ SYBR® Green Assay: PTGER3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029330

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PTGER3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PTGER3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014340
Assay Design:   Intron-spanning
Chromosome Location:   1:71478033-71512449question
Amplicon Length:   191
Splice Variants Targeted:   ENST00000370931 ENST00000460330 ENST00000356595 ENST00000306666 ENST00000370924 ENST00000370932 ENST00000370934 ENST00000354608 ENST00000351052 ENST00000414819

Gene Information

The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor may have many biological functions which involve digestion nervous system kidney reabsorption and uterine contraction activities. Studies of the mouse counterpart suggest that this receptor may also mediate adrenocorticotropic hormone response as well as fever generation in response to exogenous and endogenous stimuli. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2009]

Gene Symbol:   PTGER3
Gene Name:   prostaglandin E receptor 3 (subtype EP3)
Aliases:   EP3, EP3-I, EP3-II, EP3-III, EP3-IV, EP3e, MGC141828, MGC141829, MGC27302
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000050628
Entrez:   5733
Chromosome Mapping:   1p31.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.010000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download