PrimePCR™ SYBR® Green Assay: MCF2L, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage


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PrimePCR™ PreAmp for SYBR® Green Assay: MCF2L, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: MCF2L, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0014624
Assay Design:		Intron-spanning
Chromosome Location:		13:113669107-113679068question
Amplicon Length:		135
Splice Variants Targeted:		ENST00000375608 ENST00000397030 ENST00000430480 ENST00000421756 ENST00000433807 ENST00000409954 ENST00000375597 ENST00000397024 ENST00000442652 ENST00000375604 ENST00000535094 ENST00000375601 ENST00000434480 ENST00000423482

Gene Information

Description Not Available

Gene Symbol:		MCF2L
Gene Name:		MCF.2 cell line derived transforming sequence-like
Aliases:		ARHGEF14, DBS, FLJ12122, KIAA0362, OST
RefSeq:		NC_000013.10 NT_027140.6
Ensembl:		ENSG00000126217
Entrez:		23263
Chromosome Mapping:		13q34

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999400
y-intercept	36.280000
Efficiency	92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.