PrimePCR™ SYBR® Green Assay: IL3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0030115


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PrimePCR™ PreAmp for SYBR® Green Assay: IL3, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: IL3, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0015712
Assay Design:		Intron-spanning
Chromosome Location:		5:131396662-131398086question
Amplicon Length:		92
Splice Variants Targeted:		ENST00000296870

Gene Information

The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity and it may be associated with neurologic disorders. [provided by RefSeq Jul 2008]

Gene Symbol:		IL3
Gene Name:		interleukin 3 (colony-stimulating factor, multiple)
Aliases:		IL-3, MCGF, MGC79398, MGC79399, MULTI-CSF
RefSeq:		NC_000005.9 NT_034772.6
Ensembl:		ENSG00000164399
Entrez:		3562
Chromosome Mapping:		5q31.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998700
y-intercept	34.330000
Efficiency	102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.