PrimePCR™ SYBR® Green Assay: GNRH1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0030176


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PrimePCR™ PreAmp for SYBR® Green Assay: GNRH1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: GNRH1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0015805
Assay Design:		Intron-spanning
Chromosome Location:		8:25276899-25279123question
Amplicon Length:		83
Splice Variants Targeted:		ENST00000421054 ENST00000276414

Gene Information

The protein encoded by this gene is secreted and then cleaved to form the 10 aa luteinizing hormone-releasing hormone (LHRH) also known as gonadoliberin-1 and prolactin release-inhibiting factor also known as GnRH-associated peptide 1. LHRH stimulates the release of luteinizing and follicle stimulating hormones which are important for reproduction. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jan 2010]

Gene Symbol:		GNRH1
Gene Name:		gonadotropin-releasing hormone 1 (luteinizing-releasing hormone)
Aliases:		GNRH, GRH, LHRH, LNRH
RefSeq:		NC_000008.10 NG_016457.1 NT_167187.1
Ensembl:		ENSG00000147437
Entrez:		2796
Chromosome Mapping:		8p21-p11.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998600
y-intercept	34.500000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.