PrimePCR™ SYBR® Green Assay: PREX1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031920

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PrimePCR™ PreAmp for SYBR® Green Assay: PREX1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PREX1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018544
Assay Design:   Intron-spanning
Chromosome Location:   20:47249102-47251267question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000371941 ENST00000396220

Gene Information

The protein encoded by this gene acts as a guanine nucleotide exchange factor for the RHO family of small GTP-binding proteins (RACs). It has been shown to bind to and activate RAC1 by exchanging bound GDP for free GTP. The encoded protein which is found mainly in the cytoplasm is activated by phosphatidylinositol-345-trisphosphate and the beta-gamma subunits of heterotrimeric G proteins. [provided by RefSeq Jul 2008]

Gene Symbol:   PREX1
Gene Name:   phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1
Aliases:   KIAA1415, P-REX1
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000124126
Entrez:   57580
Chromosome Mapping:   20q13.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.320000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download