PrimePCR™ SYBR® Green Assay: ARNTL, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: ARNTL, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: ARNTL, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0023313
Assay Design:		Intron-spanning
Chromosome Location:		11:13380086-13381931question
Amplicon Length:		63
Splice Variants Targeted:		ENST00000389707 ENST00000401424 ENST00000529388 ENST00000530357 ENST00000403290 ENST00000403510 ENST00000403482 ENST00000396441 ENST00000361003 ENST00000389708 ENST00000339640

Gene Information

The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene activating this gene and possibly other circadian rhythym-associated genes. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:		ARNTL
Gene Name:		aryl hydrocarbon receptor nuclear translocator-like
Aliases:		BMAL1, BMAL1c, JAP3, MGC47515, MOP3, PASD3, TIC, bHLHe5
RefSeq:		NC_000011.9 NT_009237.18
Ensembl:		ENSG00000133794
Entrez:		406
Chromosome Mapping:		11p15

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999300
y-intercept	36.090000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.