PrimePCR™ lncRNA SYBR® Green Assay:UCP2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0180504

List Price:    $169.00
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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: UCP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: UCP2, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0103264
Assay Design:   exonic
Chromosome Location:   chr11:73976864-73976969question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000536983 ENST00000544615 ENST00000545562 ENST00000545212 ENST00000310473
LNCipedia Splice Variants Targeted:   lnc-UCP3-4:1

Gene Information

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed in many tissues with the greatest expression in skeletal muscle. It is thought to play a role in nonshivering thermogenesis obesity and diabetes. Chromosomal order is 5'-UCP3-UCP2-3'. [provided by RefSeq Jul 2008]

Gene Symbol:   UCP2
Gene Name:   uncoupling protein 2 (mitochondrial, proton carrier)
Aliases:   BMIQ4, SLC25A8, UCPH
RefSeq:   NC_000011.9 NG_011478.1 NT_167190.1
Ensembl:   ENSG00000175567
LNCipedia:   lnc-UCP3-4
Entrez:   7351
Chromosome Mapping:   11q13
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download