PrimePCR™ lncRNA SYBR® Green Assay:RET, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0180958

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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: RET, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: RET, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0103718
Assay Design:   exonic
Chromosome Location:   chr10:43077328-43100561question
Amplicon Length:   76
Refseq Transcripts Amplified:   NM_020975,NM_020630
Splice Variants Targeted:   ENST00000355710 ENST00000340058 ENST00000615310
LNCipedia Splice Variants Targeted:   lnc-CSGALNACT2-3:1

Gene Information

This gene a member of the cadherin superfamily encodes one of the receptor tyrosine kinases which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia type IIA multiple endocrine neoplasia type IIB Hirschsprung disease and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed. [provided by RefSeq Jul 2008]

Gene Symbol:   RET
Gene Name:   ret proto-oncogene
Aliases:   CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET-ELE1, RET51
RefSeq:   NC_000010.10 NT_033985.7 NG_007489.1
Ensembl:   ENSG00000165731
LNCipedia:   lnc-CSGALNACT2-3
Entrez:   5979
Chromosome Mapping:   10q11.2
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download