PrimePCR™ lncRNA SYBR® Green Assay:TP53, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0181740

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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: TP53, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: TP53, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0104500
Assay Design:   exonic
Chromosome Location:   chr17:7675098-7676393question
Amplicon Length:   120
Refseq Transcripts Amplified:   NM_001126112,NM_001276761,NM_000546,NM_001276760,NM_001126113,NM_001276695,NM_001126114,NM_001276696,NM_001126118,NM_001126115,NM_001276697,NM_001126117,NM_001276699,NM_001126116,NM_001276698
Splice Variants Targeted:   ENST00000445888 ENST00000619485 ENST00000620739 ENST00000269305 ENST00000610292 ENST00000504937 ENST00000619186 ENST00000615910 ENST00000635293 ENST00000610538 ENST00000455263 ENST00000610623 ENST00000504290 ENST00000622645 ENST00000420246 ENST00000617185 ENST00000510385 ENST00000618944 ENST00000509690 ENST00000505014 ENST00000514944 ENST00000413465 ENST00000359597
LNCipedia Splice Variants Targeted:   lnc-SAT2-3:2

Gene Information

This gene encodes tumor protein p53 which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest apoptosis senescence DNA repair or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation DNA-binding and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms which can regulate p53 transcriptional activity. [provided by RefSeq Jul 2008]

Gene Symbol:   TP53
Gene Name:   tumor protein p53
Aliases:   FLJ92943, LFS1, P53, TRP53
RefSeq:   NC_000017.10 NG_017013.1 NT_010718.16
Ensembl:   ENSG00000141510
LNCipedia:   lnc-SAT2-3
Entrez:   7157
Chromosome Mapping:   17p13.1
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download