PrimePCR™ lncRNA SYBR® Green Assay:FGF2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLIP0181105

List Price:    $169.00
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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: FGF2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: FGF2, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLID0103865
Assay Design:   intron_spanning
Chromosome Location:   chr4:122876347-122892283question
Amplicon Length:   120
Refseq Transcripts Amplified:   NM_002006
Splice Variants Targeted:   ENST00000264498 ENST00000614010 ENST00000608478
LNCipedia Splice Variants Targeted:   lnc-SPATA5-8:1

Gene Information

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes such as limb and nervous system development wound healing and tumor growth. The mRNA for this gene contains multiple polyadenylation sites and is alternatively translated from non-AUG (CUG) and AUG initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect whereas the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq Jul 2008]

Gene Symbol:   FGF2
Gene Name:   fibroblast growth factor 2 (basic)
Aliases:   BFGF, FGFB, HBGF-2
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000138685
LNCipedia:   lnc-SPATA5-8
Entrez:   2247
Chromosome Mapping:   4q26
Gene Strand:   Not Available

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download