PrimePCR™ SYBR® Green Assay: Efna5, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043711

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Efna5, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Efna5, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041495
Assay Design:   exonic
Chromosome Location:   17:62606189-62606319question
Amplicon Length:   101
Splice Variants Targeted:   ENSMUST00000069098 ENSMUST00000047379 ENSMUST00000120563 ENSMUST00000003568 ENSMUST00000064924 ENSMUST00000154755 ENSMUST00000160583 ENSMUST00000061035 ENSMUST00000034231 ENSMUST00000076840 ENSMUST00000163332 ENSMUST00000010251 ENSMUST00000170817 ENSMUST00000088452 ENSMUST00000088454 ENSMUST00000100262 ENSMUST00000037418 ENSMUST00000111829 ENSMUST00000032736 ENSMUST00000015511 ENSMUST00000046186 ENSMUST00000109445 ENSMUST00000109443 ENSMUST00000109442 ENSMUST00000109441 ENSMUST00000038004 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Efna5
Gene Name:   Ephrin A5
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000090425
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 37.730000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download