PrimePCR™ SYBR® Green Assay: Gm5506, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qMmuCIP0033613


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PrimePCR™ PreAmp for SYBR® Green Assay: Gm5506, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Gm5506, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0016311
Assay Design:		Intron-spanning
Chromosome Location:		18:48045371-48046797question
Amplicon Length:		110
Splice Variants Targeted:		ENSMUST00000076155

Gene Information

This gene may represent an evolving pseudogene of the alpha-enolase (enolase 1 alpha non-neuron) gene which has multiple pseudogenes. This gene has an intact open reading frame as well as strong transcriptional support. The length of encoded protein is conserved compared to the original enolase 1 protein. The exact function of this gene is unknown. [provided by RefSeq Jul 2008]

Gene Symbol:		Gm5506
Gene Name:		Predicted gene 5506
Aliases:		EG433182, Eno1
RefSeq:		NC_000084.6 NT_039674.8
Ensembl:		ENSMUSG00000059040
Entrez:		433182
Chromosome Mapping:		18 C\|18

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999700
y-intercept	35.980000
Efficiency	96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.