PrimePCR™ SYBR® Green Assay: Grem1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Grem1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Grem1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020761
Assay Design:   Intron-spanning
Chromosome Location:   2:113750084-113758556question
Amplicon Length:   108
Splice Variants Targeted:   ENSMUST00000095923 ENSMUST00000046751 ENSMUST00000034650 ENSMUST00000098852 ENSMUST00000030614 ENSMUST00000041516 ENSMUST00000027748 ENSMUST00000047379 ENSMUST00000120563 ENSMUST00000099575

Gene Information

Description Not Available

Gene Symbol:   Grem1
Gene Name:   Gremlin 1
Aliases:   Cktsf1b1, Drm, Grem, ld
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000074934
Entrez:   23892
Chromosome Mapping:   2 E4|2 64.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.270000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download