PrimePCR™ SYBR® Green Assay: PFKP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: PFKP, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: PFKP, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0005325
Assay Design:		Exonic
Chromosome Location:		10:3171514-3171605question
Amplicon Length:		62
Splice Variants Targeted:		ENST00000381072

Gene Information

The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-16-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene which maps to chromosome 10p is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase which map to chromosomes 12q13 and 21q22 respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4 P3L and P2L2.[supplied by OMIM Mar 2008]

Gene Symbol:		PFKP
Gene Name:		phosphofructokinase, platelet
Aliases:		FLJ40226, PFK-C, PFKF
RefSeq:		NC_000010.10 NT_008705.16
Ensembl:		ENSG00000067057
Entrez:		5214
Chromosome Mapping:		10p15.3-p15.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.997200
y-intercept	34.730000
Efficiency	95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.