PrimePCR™ SYBR® Green Assay: YAP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0040402


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PrimePCR™ PreAmp for SYBR® Green Assay: YAP1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: YAP1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0037679
Assay Design:		exonic
Chromosome Location:		11:102100525-102100633question
Amplicon Length:		79
Splice Variants Targeted:		ENST00000526343 ENST00000282441 ENST00000531439 ENST00000524575 ENST00000529029 ENST00000537274 ENST00000345877

Gene Information

This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural regulatory and signaling molecules in yeast nematode and mammals and may be involved in protein-protein interaction. [provided by RefSeq Jul 2008]

Gene Symbol:		YAP1
Gene Name:		Yes-associated protein 1
Aliases:		YAP, YAP2, YAP65, YKI
RefSeq:		NC_000011.9 NT_033899.8
Ensembl:		ENSG00000137693
Entrez:		10413
Chromosome Mapping:		11q13

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.996600
y-intercept	35.470000
Efficiency	102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.