PrimePCR™ SYBR® Green Assay: GSK3A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0050354


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PrimePCR™ PreAmp for SYBR® Green Assay: GSK3A, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: GSK3A, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0043092
Assay Design:		exonic
Chromosome Location:		19:42736297-42736761question
Amplicon Length:		89
Splice Variants Targeted:		ENST00000398249 ENST00000222330

Gene Information

This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase and transcription factors such as JUN. It also plays a role in the WNT and PI3K signaling pathways as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq Oct 2011]

Gene Symbol:		GSK3A
Gene Name:		glycogen synthase kinase 3 alpha
Aliases:		DKFZp686D0638
RefSeq:		NC_000019.9 NT_011109.16
Ensembl:		ENSG00000105723
Entrez:		2931
Chromosome Mapping:		19q13.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999300
y-intercept	36.520000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.