PrimePCR™ SYBR® Green Assay: VEGFA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050716

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: VEGFA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: VEGFA, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043454
Assay Design:   exonic
Chromosome Location:   6:43745231-43745321question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000372067 ENST00000324450 ENST00000417285 ENST00000413642 ENST00000372055 ENST00000482630 ENST00000425836 ENST00000372064 ENST00000372077 ENST00000519767 ENST00000520948 ENST00000523873 ENST00000523950 ENST00000457104 ENST00000518689 ENST00000523125 ENST00000518824 ENST00000230480 ENST00000520265 ENST00000336798

Gene Information

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects including mediating increased vascular permeability inducing angiogenesis vasculogenesis and endothelial cell growth promoting cell migration and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants encoding either freely secreted or cell-associated isoforms have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of and in-frame with the first AUG leading to additional isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   VEGFA
Gene Name:   vascular endothelial growth factor A
Aliases:   MGC70609, MVCD1, VEGF, VPF
RefSeq:   NC_000006.11 NG_008732.1 NT_007592.15
Ensembl:   ENSG00000112715
Entrez:   7422
Chromosome Mapping:   6p12

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