PrimePCR™ SYBR® Green Assay: FGA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0030820


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PrimePCR™ PreAmp for SYBR® Green Assay: FGA, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: FGA, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0016808
Assay Design:		Intron-spanning
Chromosome Location:		4:155510608-155511835question
Amplicon Length:		127
Splice Variants Targeted:		ENST00000302053 ENST00000403106 ENST00000457487

Gene Information

The protein encoded by this gene is the alpha component of fibrinogen a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading display vasoconstrictor and chemotactic activities and are mitogens for several cell types. Mutations in this gene lead to several disorders including dysfibrinogenemia hypofibrinogenemia afibrinogenemia and renal amyloidosis. Alternative splicing results in two isoforms which vary in the carboxy-terminus. [provided by RefSeq Jul 2008]

Gene Symbol:		FGA
Gene Name:		fibrinogen alpha chain
Aliases:		Fib2, MGC119422, MGC119423, MGC119425
RefSeq:		NC_000004.11 NG_008832.1 NT_016354.19
Ensembl:		ENSG00000171560
Entrez:		2243
Chromosome Mapping:		4q28

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999200
y-intercept	34.970000
Efficiency	103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.