PrimePCR™ SYBR® Green Assay: Col1a1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Col1a1, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Col1a1, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0021007
Assay Design:		Intron-spanning
Chromosome Location:		11:94936401-94937999question
Amplicon Length:		104
Splice Variants Targeted:		ENSMUST00000016673 ENSMUST00000045454 ENSMUST00000148078 ENSMUST00000141250 ENSMUST00000035548 ENSMUST00000034834 ENSMUST00000163694 ENSMUST00000070163 ENSMUST00000025956 ENSMUST00000112329 ENSMUST00000030765 ENSMUST00000167634 ENSMUST00000028161 ENSMUST00000023666 ENSMUST00000117099 ENSMUST00000110085 ENSMUST00000048544 ENSMUST00000110084 ENSMUST00000080300 ENSMUST00000092566 ENSMUST00000033671 ENSMUST00000001547

Gene Information

Description Not Available

Gene Symbol:		Col1a1
Gene Name:		Collagen, type I, alpha 1
Aliases:		Col1a-1, Cola-1, Cola1, Mov-13, Mov13
RefSeq:		NC_000077.6 NT_096135.6
Ensembl:		ENSMUSG00000001506
Entrez:		12842
Chromosome Mapping:		11 D\|11 56.0 cM

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.560000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.