PrimePCR™ SYBR® Green Assay: FGFR4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: FGFR4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FGFR4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0001143
Assay Design:   Exonic
Chromosome Location:   5:176522358-176522543question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000292408 ENST00000393648 ENST00000502906 ENST00000393637 ENST00000511076 ENST00000292410 ENST00000377207

Gene Information

The protein encoded by this gene is a member of the fibroblast growth factor receptor family where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region composed of three immunoglobulin-like domains a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors setting in motion a cascade of downstream signals ultimately influencing mitogenesis and differentiation. The genomic organization of this gene compared to members 1-3 encompasses 18 exons rather than 19 or 20. Although alternative splicing has been observed there is no evidence that the C-terminal half of the IgIII domain of this protein varies between three alternate forms as indicated for members 1-3. This particular family member preferentially binds acidic fibroblast growth factor and although its specific function is unknown it is overexpressed in gynecological tumor samples suggesting a role in breast and ovarian tumorigenesis. [provided by RefSeq Jul 2008]

Gene Symbol:   FGFR4
Gene Name:   fibroblast growth factor receptor 4
Aliases:   CD334, JTK2, MGC20292, TKF
RefSeq:   NC_000005.9 NT_023133.13 NG_012067.1
Ensembl:   ENSG00000160867
Entrez:   2264
Chromosome Mapping:   5q35.1-qter

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.000000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download