PrimePCR™ SYBR® Green Assay: ADD1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: ADD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ADD1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0001960
Assay Design:   Exonic
Chromosome Location:   4:2877709-2877805question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000511797 ENST00000513328 ENST00000508277 ENST00000503455 ENST00000355842 ENST00000398123 ENST00000398129 ENST00000264758 ENST00000446856 ENST00000398125 ENST00000398124

Gene Information

Adducins are a family of cytoskeleton proteins encoded by three genes (alpha beta gamma). Adducin is a heterodimeric protein that consists of related subunits which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however not all variants have been fully described. [provided by RefSeq Jul 2008]

Gene Symbol:   ADD1
Gene Name:   adducin 1 (alpha)
Aliases:   ADDA, MGC3339, MGC44427
RefSeq:   NC_000004.11 NT_006051.18 NG_012037.1
Ensembl:   ENSG00000087274
Entrez:   118
Chromosome Mapping:   4p16.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.480000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download